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Elizabet Sowden

Woont in Essen, Germany · Geboren op September 22, 1980
Algemene Informatie
In an open relationship
Birth Date:
September 22, 1980
Laatste login:
November 27, 2017
Lid sinds:
November 27, 2017
Registered User
About Me

A substantial proportion of the swine developed in North America now are created utilizing artificial Therefore, the capability to preserve animal
reproductive samples, such as semen, oocytes and embryos, for use in artificial insemination and other reproductive processes is Without the need of this capability, it is not doable to transport animal reproductive samples or retailer the samples
for any length of time as a result, the utility of the samples is limited.
To protect reproductive samples from bacterial contamination, it has been prevalent practice to introduce antibiotics into the preservation mediums or extender solutions.
Even though standard antibiotics can be a low expense and effective way of preserving the samples, there are extended term consequences of the
continued widespread use of antibiotics. Over time, bacteria come to the fore which are resistant to antibiotics.

The presence of low levels of antibiotics in agricultural livestock and human meals goods is viewed as to be one particular result in of the increasingly resistant bacteria.
The increased resistance of bacteria to antibiotics poses a danger to humans and animals as infectious illnesses are becoming extra
tough to treat. Therefore, in certain regions, conventional antibiotics are no longer helpful.
In addition, there is consideration getting offered by regulatory bodies to
banning the general use of antibiotics in animals raised for meals production. As a result, it is desirable to have an extender that has antimicrobial properties, but is
substantially free of antibiotics. It is also
desirable to have a system for preserving animal reproductive samples that uses an extender that has antibicrobial properties,
but is substantially free of charge of antibiotics.

The system of this invention mixes a colloidal suspension of silver as a element of a preservation medium for the preservation of animal reproductive samples.
By adding the colloidal suspension of silver to conventional semen extenders, for instance,
an extender for boar semen which has antimicrobial properties, but substantially
free of charge of antibiotics is created. A colloidal silver remedy possessing
a silver concentration of at least about 1×10−9 moles/liter has been identified productive in preserving some reproductive supplies.
It is an object of the present invention to present an extender with antibacterial and antiviral properties without the
need of the use of antibiotics. Further objects, functions and benefits of the invention will be apparent
from the following detailed description when taken in conjunction with the accompanying drawings.

A colloidal dispersion of nanoparticles of silver may perhaps be made use of to preserve reproductive samples such
as boar semen, producing a medium which is antibacterial and antiviral.
The colloidal dispersion of silver might be produced via the approaches detailed beneath, or any
other suitable process. In certain, a colloidal silver option is mixed with an extender concentrate to kind
an extender composition. The colloidal silver solution has a silver concentration of at least about 1×10−9 moles/liter.
The silver may perhaps be present in a single or far more types, which includes ionic,
elemental, or a mixture thereof. Further, the ions might be present
in one or much more oxidation states. The silver
ions have an affinity to sulfhydryl groups in enzyme systems, and by means of
which they interfere with the transmembraneous energy transfer in bacterial microorganisms.

The extender composition is then mixed with an animal reproductive
sample to form a biological culture medium valuable for
animal reproduction. The animal reproductive sample is
ordinarily semen, oocytes, embryos or other options
usable for reproductive purposes. Preferably, the animal reproductive
sample is boar semen. The concentration and viability of the animal reproductive sample is determined by known techniques in the art.
Based on the concentration and viability of the animal reproductive sample, an amount of the
extender composition is added to maximize the quantity of doses of the biological
culture medium beneficial for animal reproduction. The quantity of
extender composition to be added to the animal reproductive sample is
determined primarily based on classic strategies in the art.
As is nicely identified, the chemical composition of animal reproductive samples will
differ from animal to animal, and even between collections from the similar

Excessive levels of chlorine or nitrogen in the sample, for example
boar semen, will kind compounds with the silver resolution which are detrimental to the effectiveness of the extender, and
at times even spermicidal. Therefore, the extender composition of the present invention must not be used where
chlorine concentrations are far more than about 700 ppm.
Likewise, if the nitrogen levels in the animal reproductive sample are too higher, the
extender composition of the present invention does not work.

The biological culture medium of the present invention is microbiologically stable at refrigerated circumstances (significantly less than about 40° F.) for up to 1
week. Four bacterial strains have been grown overnight in trypticase soy broth (TSB) at space temperature.

The next day they had been transferred to TSB (diluted 1:
100 in distilled water) and grown overnight at space temperature.
The stains have been pooled and inoculated into water from Aldrich Chemical Co.
(32,007-2 ACS grade). Bacterial stains made use of have been two Pseudomonas fluorescens a
Salmonella typhimurium and an Etrobacter agglomerans. Just after plasma-treatment, the samples have been straight plated or diluted in phosphate buffered saline and then plated on Trypticase soy agar.
The plated were incubated at room temperature for 72 hours.
To test for injured bacteria, .5 ml of each sample was added to four.5 ml of SB and
incubated at room In all solutions resulting from the plasma-remedies the bacteria were completely killed.
Even options prepared from 200 ml, initial "living-bacterial soup" and
1 ml of solution of the ten seconds plasma-treated bacterial soup exhibited a 99% reduction of the living
bacterial content.

The only samples that had been constructive for development upon enrichment have been the untreated
water and untreated water with 1 ml of 10 seconds treated
bacterial soup. Treatment of the samples,
even for five seconds, killed the bacterial No bacteria had been recovered either
by direct plate count or by enrichment. Minimizing the silver particle sizes is believed
to be critical both from the stability of the colloidal suspension and for the efficacy against
microbes. Several processes to generate nanoparticles are known. For instance, those disclosed in U.S. Nos.
five,543,133 5,585,020 five,879,750 and six,540, CS Pro Systems advertises
a high voltage AC processor creating nanoparticles of colloidal metal.

X Colloidal Silver, Copper & Gold 500ml + Totally free Pure Copper Bracelet
Purified (distilled) water
When Technologies Fails: Make Your Own Footwear
2 Silver Rods .9999 pure, approx. six" length
Take ½ to 1 cup a day internally
Saw or Swiss army knife for cutting wood strips

The HVAC approach is claimed to produce particle sizes in between .002 to .007-9 microns by imposing an AC potential of ten,000 volts across two metal electrodes in a distilled water medium. Colloidal suspensions of nanoparticles of electrically conductive materials can also be produced by generating a plasma reaction amongst two electrodes, comprising the preferred electrically conductive material(s), which are immersed within a dense fluid medium. Preferably, the dense medium is quickly recirculated between the two electrodes. For the present invention, the electrodes are silver. Usually, the electrically conducting material ought to be at least about 90 percent pure, preferably at least about 95 % pure, far more preferably at least about 99 percent pure.

Preferably, the electrodes are constructed so as to be conveniently removed and installed. This simple interchangeability facilitates replacing worn electrodes or altering electrodes to accommodate the production of diverse colloidal dispersions. The dense medium may well be any liquid having a viscosity low adequate to permit fast circulation of the fluid among the two electrodes. The plasma reaction will decompose the molecules of the dense medium into highly reactive totally free radicals. As such, the reaction products formed from the dense medium absolutely free radicals may perhaps be undesirable contaminants to the colloidal remedy. Preferably, the dense medium will not kind undesirable by-products for the duration of the plasma reaction. An undesirable by-product is any compound that must be removed, due to technical, sensible or aesthetic motives, from the colloidal dispersion prior to use. In most circumstances, when inorganic/organic hybrid nanoparticle systems are prepared, liquid phase organometallic compounds can be utilised.

The dense medium is ordinarily chosen to prevent the production of undesirable byproducts. OH−) readily react with every single other to reform the water molecule. In contrast, the decomposition reaction products of other dense media, e.g. benzene, are no cost radicals which may initiate polymerization reactions. The dense medium, and any by-solution of the plasma reaction, really should have, at most, a slow reaction price with the electrically conductive material. Preferably, any reaction amongst the dense medium and the electrically conductive material is slow sufficient that the nanoparticles in the colloidal dispersion have the desired shelf-life. Most preferably, the dense medium, and any by-product, is non-reactive with the electrically conducting material.